
Manuel Leonetti, Ph.D.
Group Leader
B.Sc. Ecole Normale Superieure, Paris - Chemistry
M.Sc. Ecole Normale Superieure, Paris - Interdisciplinary Life Sciences
Ph.D. The Rockefeller University - Molecular Neurobiology
Hera Canaj
Research Associate
B.Sc. University of Western Ontario - Genetics
Joana Cabrera
Associate R&D Engineer
B.Sc. Univ. of California, Berkeley - Bioengineering
Nathan Cho
Research Associate
B.Sc. Stanford University - Biology
Jason (Yucheng) Li
Research Associate
B.Sc. Harvard University - Molecular and Cell Biology
Preethi Raghavan
Research Associate
B.Sc. Stanford University - Bioengineering
Laura Savy
Research Associate
B.Sc. Institut National de Sciences Appliquees, France - Biochemistry
M.Sc. Institut National de Sciences Appliquees, France - Molecular Biology
Partner
Dan Itzhak, Ph.D.
Cell Atlas Proteomics Fellow
B.A. University of Cambridge, UK - Genetics
M.Phil. University of Cambridge, UK - Biological Science
Ph.D. The Institute of Cancer Research, UK - Biology
Visiting students
Rachel Brunetti
Ph.D. candidate, University of California, San Francisco (Orion Weiner’s lab)
Christian Gnann
M.Sc. candidate, KTH Royal Institute of Technology, Sweden
Abimbola Jarvis, Ph.D.
Biotechnology program, City College of San Francisco
Our Science
Illuminating Cellular Architecture
We are interested in describing and understanding how human cells are built as ecosystems of molecular machines. We are motivated by how this will help characterizing fundamental mechanisms in physiology and diseases, but also by the process of “reverse engineering” a cell - understanding the details of how it is built to be able to predictably tune its properties and behavior.
To start, we are asking: Where are all the proteins in a cell localized, and how do they interact with each other? For this, we develop and deploy a variety of technologies to “illuminate” proteins and manipulate them:
- We specialize in the development of scalable CRISPR gene editing methods to create genome-scale libraries of fluorescently labeled cell lines.
- We build software and hardware automation to streamline the design, execution and analysis of our experiments.
- We use high-throughput live cell fluorescence microscopy to map protein localization in space and time.
- We use proteomics mass-spectrometry to profile protein-protein interactions and delineate functional networks.
Our goal is to build open datasets, software and protocols for the entire community to use and explore.
More about our project in this recent talk:
Our Publications
You can view our publications here.