ILLUMINATING CELLULAR ARCHITECTURE
We are interested in describing and understanding how human cells are built as ecosystems of molecular machines. We are motivated not only by how this will help characterize fundamental mechanisms in physiology and diseases, but also by the process of “reverse engineering” a cell – understanding the details of how it is built to be able to predictably tune its properties and behavior.
To start, we are asking: Where are all the proteins in a cell localized, and how do they interact with each other? For this, we develop and deploy a variety of technologies to “illuminate” proteins and manipulate them:
- We specialize in the development of scalable CRISPR gene editing methods to create genome-scale libraries of fluorescently labeled cell lines.
- We build software and hardware automation to streamline the design, execution, and analysis of our experiments.
- We use high-throughput live cell fluorescence microscopy to map protein localization in space and time.
- We use proteomics mass-spectrometry to profile protein-protein interactions and delineate functional networks.
Our goal is to build open datasets, software, and protocols for the entire community to use and explore.
More about our project in this recent talk
You can view our publications here.