Intracellular Architecture

Manuel Leonetti, Ph.D.

Group Leader
B.Sc. Ecole Normale Superieure, Paris - Chemistry
M.Sc. Ecole Normale Superieure, Paris - Interdisciplinary Life Sciences
Ph.D. The Rockefeller University - Molecular Neurobiology

Hera Canaj

Research Associate
B.Sc. University of Western Ontario - Genetics

 

Joana Cabrera

Associate R&D Engineer
B.Sc. Univ. of California, Berkeley - Bioengineering

 

Nathan Cho

Research Associate
B.Sc. Stanford University - Biology

 

Jason (Yucheng) Li

Research Associate
B.Sc. Harvard University - Molecular and Cell Biology

 

Preethi Raghavan

Research Associate
B.Sc. Stanford University - Bioengineering

 

Laura Savy

Research Associate
B.Sc. Institut National de Sciences Appliquees, France - Biochemistry
M.Sc. Institut National de Sciences Appliquees, France - Molecular Biology

 

Partner

Dan Itzhak, Ph.D.

Cell Atlas Proteomics Fellow
B.A. University of Cambridge, UK - Genetics
M.Phil. University of Cambridge, UK - Biological Science
Ph.D. The Institute of Cancer Research, UK - Biology

 

Visiting students

Rachel Brunetti

Ph.D. candidate, University of California, San Francisco (Orion Weiner’s lab)

 

 

Christian Gnann

M.Sc. candidate, KTH Royal Institute of Technology, Sweden

 

 

Abimbola Jarvis, Ph.D.

Biotechnology program, City College of San Francisco

 

 

Our Science

Illuminating Cellular Architecture

We are interested in describing and understanding how human cells are built as ecosystems of molecular machines. We are motivated by how this will help characterizing fundamental mechanisms in physiology and diseases, but also by the process of “reverse engineering” a cell - understanding the details of how it is built to be able to predictably tune its properties and behavior.

To start, we are asking: Where are all the proteins in a cell localized, and how do they interact with each other? For this, we develop and deploy a variety of technologies to “illuminate” proteins and manipulate them:

  • We specialize in the development of scalable CRISPR gene editing methods to create genome-scale libraries of fluorescently labeled cell lines.
  • We build software and hardware automation to streamline the design, execution and analysis of our experiments.
  • We use high-throughput live cell fluorescence microscopy to map protein localization in space and time.
  • We use proteomics mass-spectrometry to profile protein-protein interactions and delineate functional networks.

Our goal is to build open datasets, software and protocols for the entire community to use and explore.

More about our project in this recent talk:

 

Our Publications

You can view our publications here.