Resources
The Rapid Response Team provides metagenomic next-generation sequencing (mNGS) training and support to groups all over the world. Here you will find mNGS protocols and training materials that were developed in collaboration with the Genomics and Sequencing Platform at CZ Biohub and the CZ ID team at the Chan Zuckerberg Initiative.
Lab Protocols
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Metagenomic next-generation sequencing (mNGS) protocols.
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SARS-CoV-2 metagenomic sequencing by spiked primer enrichment (MSSPE), library prep protocol.
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SARS-CoV-2 ARTIC sequencing using Illumina, library prep protocol.
Training Materials
A. Metagenomic Sequencing
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Metagenomic sequencing (mNGS): introduction and lab setup.
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mNGS: study design and sample preparation.
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Introduction to Illumina sequencing and data analysis for mNGS using IDseq.
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Library preparation for mNGS.
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Loading the sequencer.
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iSeq loading guide.
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MiSeq loading guide.
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iSeq loading guide.
B. Enrichment/Depletion Methods for Sequencing
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FLASH protocol.
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FLASH.
C. Additional material
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How to make your own SPRI beads.
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Sequencing SARS-CoV-2 for public health laboratories.
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Scaling sequencing.