The Rapid Response Team provides metagenomic next-generation sequencing (mNGS) training and support to groups all over the world. Here you will find mNGS protocols and training materials that were developed in collaboration with the Genomics and Sequencing Platform at CZ Biohub and the CZ ID team at the Chan Zuckerberg Initiative.

Lab Protocols

  • Metagenomic next-generation sequencing (mNGS) protocols.
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  • SARS-CoV-2 metagenomic sequencing by spiked primer enrichment (MSSPE), library prep protocol.
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  • SARS-CoV-2 ARTIC sequencing using Illumina, library prep protocol.
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Training Materials

A. Metagenomic Sequencing

B. Enrichment/Depletion Methods for Sequencing

C. Additional material