Nextseq and Novaseq – 2 dye
- Rather than a separate dye for each base, 2-channel SBS uses a mix of dyes. Images are taken of each DNA cluster using red and green wavelength filter bands.
- Clusters seen in red or green images are interpreted as C and T bases, respectively. Clusters observed in both red and green images are flagged as A bases (appearing as yellow clusters), while unlabeled clusters are identified as G bases).
- Miseq – 4 dyes/2 lasers
- G/T share one laser and C/A share another. When choosing indexes, make sure each base has diversity between the two lasers.
- One dye and two chemistry step