Manuel Leonetti‘s, team brings together cell biologists, data scientists, and engineers to build a reference map of how the human cell is internally organized. Their flagship project, OpenCell, is combining CRISPR engineering, live-cell microscopy, and proteomics to characterize the spatial location and molecular interactions of all the proteins that make up the cell.
Quantitative Cell Science
The maps we need
Diseases are caused by disruptions in the inner workings of cells or in the communication between cells. That’s why we support rigorous, quantitative research in cell biology and developmental biology, showing how healthy cells work and, more importantly, what takes place when disease strikes.
Malfunctioning cells are at the root of many human diseases, including cardiovascular disease, neurodegeneration, diabetes, and cancer. Our goals are to map and understand cellular physiology across scales in time and space and to make the data we obtain openly available to advance all research in cell biology. Understanding the healthy cell is a prerequisite to diagnosing, understanding, and treating the diseased state.
Using state-of-the-art, custom-built light-sheet microscopes coupled with spatial transcriptomics and photoactivation of lineage-specific reporters, Loïc Royer and his group are mapping how tissues form during early development at unprecedented spatial and temporal resolution. These studies are enabled by innovative tools for machine learning, image analysis, refinement, and visualization.
Keir Balla‘s group, which straddles Infectious Disease and Quantitative Cell Science, discovers zebrafish viruses and deploys them as tools for capturing infection dialogs in toto with visual and molecular precision.
Led by Greg Huber, a group of mathematicians and computational and theoretical physicists is working in close collaboration with experimental biologists, using tools from applied mathematics and theoretical physics to understand problems in cell biology and beyond.
Adrian Jacobo‘s group uses a combination of zebrafish transgenesis, cutting-edge imaging tools, and mathematical modeling to understand how tissue architecture emerges from the spatial and temporal dynamics of cell-cell signaling.
Ranen Aviner’s group uses molecular biology, transcriptomics, and proteomics to investigate how ribosomal networks change during viral infection. Aviner also works in the Biohub’s Quantitative Cell Science initiative on neurodegeneration, with the goal of designing rational, therapeutic interventions.
At the center of CZ Biohub’s Quantitative Cell Science efforts are the Tabula projects led by CZ Biohub Network President Steve Quake and collaborators, who apply single-cell transcriptomics and data sciences to identify the thousands of cell types that comprise the tissues and organs in whole organisms, from fruit flies (Tabula Drosophilae) to mice (Tabula Muris) to humans (Tabula Sapiens).
OpenCell – A Collection of Localization and Interactome Measurements for Human Proteins
A collaboration between the Leonetti group at CZ Biohub and the Mann Lab at the Max Planck Institute of Biochemistry, along with many other colleagues.